bidirectional teto enhancer promoter  (Addgene inc)

Journal: bioRxiv

Article Title: Increased Complement C4 in a Sparse Neuronal Subset Induces Network-Wide Transcriptomic Alterations in the Prefrontal Cortex

doi: 10.1101/2025.05.29.656749

Figure Lengend Snippet: ( A ) IUE was performed on gestation day 16 (E16) CD01 dams (Cartoon adapted from Phadke et al. (2024). In control conditions, we expressed GFP under the strong CAG promoter. In the C4-OE condition, we electroporated two plasmids under the CAG promoter to express GFP and overexpress mouse C4 ( mC4, C4b, or C4 ). All plasmids were expressed at a final concentration of 1 μg/μl. ( B ) Cartoon and slices of the P21-23 PFC containing cells that have been isolated using microdissection (yellow dotted line). scale bar = 100 μm. ( C ) After microdissection, mPFC tissue was immediately flash-frozen on dry ice. Next, cDNA library preparation was performed by the Broad Genomics Platform using the Illumina TruSeq™ Stranded mRNA Sample Preparation Kit (isolating at least 250 ng of purified total RNA). Whole transcriptome sequencing was performed at the Broad Institute Genomic Services, and R (v4.2.1) was used for further analysis.

Article Snippet: For GFP control, we used a plasmid containing EGFP under the CAG promoter (pCAG-EGFP, Addgene plasmid #11150; http://n2t.net/addgene:11150;RRID:Addgene_11150 ) ( ).

Techniques: Control, Concentration Assay, Isolation, Laser Capture Microdissection, cDNA Library Assay, Sample Prep, Purification, Sequencing